Composite

Part:BBa_K2760033:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-14)


Lactobachill Plan A (L.rhamnosus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1970
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal AgeI site found at 844
    Illegal AgeI site found at 2681
    Illegal AgeI site found at 2793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 776
    Illegal BsaI.rc site found at 1572
    Illegal SapI.rc site found at 1589
    Illegal SapI.rc site found at 1809


Design Notes

    • Designed for L. rhamnosus GG


Source

It contains the the spaCBA promoter (BBa_K2760005), a L. rhamnosus GG ribosome binding site (BBa_K2760006), then the codon optimized sequence for protein nsrR (BBa_K2760023) which is the PyeaR promoter repressor, two stop codons and then another ribosome binding site (the same as earlier). After this ribosome binding site, follows a modified and optimized iLOV sequence (BBa_K2760024), two stop codons and a loop terminator (BBa_K2760007). A PyeaR promoter follows (BBa_K216005), the same ribosome binding site, the signal peptide 1, SP1-NSP4 (BBa_K2760000), afterwards, the gp130 (receptor IL-6 complex) human soluble protein (BBa_K2760002) and a His-Tag (BBa_K2760012), then two stop codons, the RBS and finally an optimized M-cherry (BBa_K2760029), stop codon, loop terminator 908 (BBa_K2760008).

References